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  • 蜂巢矩陣葉綠素熒光成像系統HEXAGON-IMAGING-PAM
    日期:2022-08-31 09:30:40

    蜂巢矩陣葉綠素熒光成像系統

    HEXAGON-IMAGING-PAM

    葉綠素熒光成像系統的六邊形戰士

    精度高,面積大,功能全,應用廣,文獻多,數據可視化!

    header-hexagon-imaging-pam-2560x1440-1086f31d.jpg

    HEXAGON-IMAGING-PAM是德國WALZ公司新推出的大型蜂巢矩陣葉綠素熒光成像系統。它憑借高精度的脈沖振幅調制(PAM)技術,可以對20×24cm的區域進行成像。分辨率高達1.2 MP(1000 x 1200 px, 2x2 binning技術,實際是2000×2400),像素尺寸3.45 x 3.45 μm。

    超高分辨率的基礎是成像區域光場的均勻性,在設計過程中,光源陣列中LED的位置是經過精心布局的,以保證測量區域內無陰影,所有成像區域內的樣品均勻照光,樣品間的差異可以盡收眼底。大功率LED面板的冷卻效果非常好,可以大限度的延長LED的使用壽命。

    增加遠紅光(FR)LED 面板,可用于測量所研究樣品的Fo'值。

    HEXAGON-IMAGING-PAM采用蜂巢矩陣式LED面板拼接技術,單個六邊形蜂巢矩陣單元之間LED的不平橫可以獨立補償,初衷是為實現樣品區域的理想照明提供更優的選擇。

    盡管成像區域很大,但是它依然足夠靈活,可以測量各種類型的樣品,如盆栽植物,穴盤中培養的植物,培養皿上的植物或多孔板中的藻類懸浮液。

    滑動門設計,集成安全關閉功能,開門狀態下,飽和脈沖的強度會被抑制以保護操作人員的眼睛。

    主要功能

    l 原位測量:活體植物葉綠素熒光成像,直觀顯示樣品光合作用光能利用差異,可導出彩色圖像。

    l 成像功能:對Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、PS/50=ETR、Inh等參數進行成像分析。測定調節性能量耗散Y(NPQ),反映植物光保護能力,測定非調節性能量耗散Y(NO),反映植物光損傷程度。

    l 程序測量功能:可自動程序測量熒光誘導曲線、快速光曲線和暗弛豫,也可手動測量;在測量過程中能自動分析所有熒光參數的變化趨勢;可以預編程進行自定義實驗流程,如模擬波動光。

    l AOI功能:可在測量前或測量后任意選擇感興趣的區域(AOI),程序將自動對選擇的AOI的數據進行變化趨勢分析,并在報告文件中顯示相關AOI的數據。所有報告文件中顯示的數據都可導出到EXCEL文件中。

    l 成像異質性分析功能:對任意參數任意時間的成像,可在圖像上任意選取兩點,軟件自動對兩點間的數據進行橫向異質性分析,并可導出到EXCEL文件中。

    l 成像數據范圍分析功能:對任意參數任意時間的成像,可分析任意兩個熒光數值之間有多少個像素點,多少面積(cm2。

    l 突變株篩選功能:可跟據成像結果快速篩選光合、產氫/油、抗逆(抗鹽、抗旱、抗病等)等突變株。

    l 微藻毒理研究功能:可同時測量496孔板,即384個微藻樣品(對照和處理組)的光合活性,軟件自動給出處理組樣品相對于對照組的光合抑制百分比。

    7599516cab1fcc6b75f0f9ceaa70ce5.png

    應用領域

    l 光合作用研究:可以在完全相同的條件下同時對大量樣品進行成像

    l 植物病理學:病斑部位(包括肉眼不可見時)成像以及病斑擴散的時空動力學

    l 植物脅迫生理學:肉眼不可見生物/非生物脅迫損傷的早期檢測

    l 遺傳育種:出苗后大規??焖俸Y選高光合/抗旱/抗熱/抗凍/抗病等植株

    l 突變株篩選:快速篩選模式植物的光合突變株、抗逆突變株、產氫微藻突變株等

    l 微藻毒理學:不同毒物濃度多個重復的樣品一次測完,軟件自動計算抑制比率

    l 其它多種擴展研究

    成像參數

    Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), PS/50=ETR,Inh.

    imagingwingige-software-screenshot-753f5098.png


    蜂巢矩陣葉綠素熒光成像系統視頻二維碼.png

    產地:德國WALZ


    參考文獻

    數據來源:光合作用文獻Endnote數據庫,原始數據來源:Google Scholar。

    注:HEXAGON-IMAGING-PAM為新產品,暫無文獻發表,研究成果可參考M-IMAGING-PAM發表文章。

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